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Isolement humain de neutrophile de sang périphérique pour interroger la voie de kinase LRRK2 associée de Parkinson en évaluant la phosphorylation de Rab10
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JoVE Journal Bioquímica
Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation
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Isolement humain de neutrophile de sang périphérique pour interroger la voie de kinase LRRK2 associée de Parkinson en évaluant la phosphorylation de Rab10

7,199 Views

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12:49 min

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March 21, 2020

DOI:

10.3791/58956-v

DOI:
10.3791/58956-v

•

12:49 min
March 21, 2020

•

7 Views
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1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2The Michael J. Fox Foundation for Parkinson’s Research, 3Department of Medical and Molecular Genetics,Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson’s Disease, Toronto Western Hospital,University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee

Summary

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Les mutations dans le gène riche de leucine de répétition de kinase 2 (LRRK2) causent la maladie héréditaire de Parkinson. Nous avons développé une méthode facile et robuste pour évaluer la phosphorylation LRRK2-contrôlée de Rab10 dans les neutrophiles périphériques humains de sang. Ceci peut aider à identifier des individus avec l’activité accrue de voie de kinase de LRRK2.

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Cite this Article

Fan, Y., Tonelli, F., Padmanabhan, S., Baptista, M. A., Riley, L., Smith, D., Marras, C., Howden, A., Alessi, D. R., Sammler, E. Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation. J. Vis. Exp. (157), e58956, doi:10.3791/58956 (2020).

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