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A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
This content is Free Access.
1Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert,INRA, 4Department of Cell Biology and Molecular Genetics,University of Maryland
Capítulos
- 00:04Título
- 00:31Designing and Preparing the Peptides
- 02:06Preparing the Fluorescence Plate Reader
- 03:11Preparing the Assay
- 04:30Data Analysis
- 05:37Results: Mutations in the Cleavage Site Alter Activity of Furin and Cleavage Efficiency
- 07:26Conclusion
We present a fluorogenic peptide cleavage assay that allows a rapid screening of the proteolytic activity of proteases on peptides representing the cleavage site of viral fusion peptides. This method can also be used on any other amino acid motif within a protein sequence to test for the protease activity.
