Journal
/
Pesquisa do Câncer
/
Rilevazione in tempo reale nell'apoptosi delle cellule tumorali in Vitro indotta da CD8+ T cellule per studiare funzioni immuni soppressive di tumore-infiltrazione di cellule mieloidi
JoVE Journal
Pesquisa do Câncer
This content is Free Access.
JoVE Journal Pesquisa do Câncer
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
Please note that all translations are automatically generated. Click here for the English version.

Rilevazione in tempo reale nell'apoptosi delle cellule tumorali in Vitro indotta da CD8+ T cellule per studiare funzioni immuni soppressive di tumore-infiltrazione di cellule mieloidi

21,531 Views

•

09:57 min

•

January 29, 2019

DOI:

10.3791/58841-v

DOI:
10.3791/58841-v

•

09:57 min
January 29, 2019

•

7 Views
, , ,
1Royal (Dick) School of Veterinary Studies and Roslin Institute,University of Edinburgh, 2MRC Centre for Reproductive Health,University of Edinburgh, 3Edinburgh Phenotypic Assay Centre,University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine,University of Edinburgh

Summary

Automatically generated

Descriviamo qui un protocollo per studiare la citotossicità di pre-attivato CD8+ T cellule contro le cellule tumorali dalla rilevazione apoptotic microscopia del cancro cellule via in tempo reale. Questo protocollo può studiare i meccanismi dietro la soppressione delle cellule T indotta da cellula mieloide e valutare composti puntati replenishing T cellule tramite blocco delle cellule mieloidi soppressive immuni.

Explore More Videos

Tumor Cell ApoptosisCD8+ T CellsTumor-infiltrating Myeloid CellsImmune SuppressionCancer ImmunologyAutoimmune DiseasesCell-to-cell InteractionsCheckpoint InhibitorCell CultureTarget CellsT-cell Activation

Read Article

Cite this Article

Kitamura, T., Doughty-Shenton, D., Pollard, J. W., Carragher, N. O. Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells. J. Vis. Exp. (143), e58841, doi:10.3791/58841 (2019).

Download .ris file

Copy

Share Video

.

Copy