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细菌病原体在细胞和有机水平宿主反应探针中的应用
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
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细菌病原体在细胞和有机水平宿主反应探针中的应用

DOI:
10.3791/58775-v

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08:38 min

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February 22, 2019

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, , ,
1Department of Microbiology & Immunology,University of Miami Miller School of Medicine, 2Department of Microbiology & Immunology,University of Illinois College of Medicine

Capítulos

  • 00:04Título
  • 01:08Infection Procedures
  • 02:16Sampling Processing of Intracellular and Emergent Lm for Colony-forming Unit (CFU) Assay
  • 03:58Sampling Processing of Intracellular and Emergent Lm for Flow Cytometry (FCM)
  • 06:22Results: The Role of Pathogen and Host-encoded Factors on Cellular Infection
  • 07:54Conclusion

Summary

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我们描述了可用于分析主机编码因子活性的体外和体内感染检测。

Tags

Bacterial PathogenHost ResponseIntracellular ReplicationPathogen EffluxInfection ExperimentGentamicinCell LysisDilutionAgar Plating

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