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crispr 介导的人类真菌病原体白色念珠菌基因组编辑
JoVE Journal
Genética
This content is Free Access.
JoVE Journal Genética
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
DOI:

09:56 min

November 14, 2018

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Capítulos

  • 00:04Título
  • 00:27Identification and Cloning of Guide RNA Sequence
  • 04:28Designing and Generation of Repair Template
  • 05:33Transformation of C. albicans with Repair Template and Plasmid
  • 07:47Colony PCR
  • 08:52Results: Evaluation of Successful Genome Editing in C. albicans
  • 09:27Conclusion

Summary

Tadução automática

白色念珠菌的高效基因组工程是了解治疗方法的发病机制和发展的关键。在这里, 我们描述了一个协议, 以快速和准确地编辑白色念珠菌基因组使用 crispr. 该协议允许调查人员引入各种各样的基因修饰, 包括点突变、插入和删除。

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