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Transkripsiyon faktörü-DNA bağlayıcı Affinities floresans mikroskobu kullanarak rekabet titrasyon tarafından yüksek hassasiyet ölçümü

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

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Transkripsiyon faktörü-DNA bağlayıcı Affinities floresans mikroskobu kullanarak rekabet titrasyon tarafından yüksek hassasiyet ölçümü

8,375 Views

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06:38 min

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February 07, 2019

DOI:

10.3791/58763-v

DOI:

10.3791/58763-v

•

06:38 min

February 07, 2019

•

2 Views

Christophe Jung, Max Schnepf, Peter Bandilla, Ulrich Unnerstall, Ulrike Gaul

¹Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM),Ludwig-Maximilians-Universität München

Tags

Transcription Factor-DNA Binding Fluorescence Microscopy Dissociation Constant Competitive Titration Anisotropy Measurement Binding Affinity Protein-DNA Interaction DNA Annealing Agarose Gel Titration Plate Calibration Well Nile Blue Dye

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