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增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多
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JoVE Journal Imunologia e Infecção
Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis
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增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多

7,095 Views

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12:35 min

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January 04, 2019

DOI:

10.3791/58751-v

DOI:
10.3791/58751-v

•

12:35 min
January 04, 2019

•

14 Views
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1Anesthesiology Department,The Second Hospital of Dalian Medical University, 2Scientific Research Center,The Second Hospital of Dalian Medical University

Tags

EGFP-expressing Escherichia ColiPhagocytosisMacrophagesPeritoneal MacrophagesInnate Immune FunctionAgingFluorescence MicroscopyFlow CytometryTA CloningPET SUMO VectorBL21 Competent CellsKanamycin

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Cite this Article

Zhang , Y., Wang, G., Lu, J., Xu, L., Xiong, J. Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis. J. Vis. Exp. (143), e58751, doi:10.3791/58751 (2019).

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