Journal
/
/
神経科学の TEM セクションをサンプリングの公平なアプローチ
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
An Unbiased Approach of Sampling TEM Sections in Neuroscience
DOI:

10:56 min

April 13, 2019

, , , , , , , , , , , ,

Capítulos

  • 00:04Título
  • 01:02TEM Preparation Embedding, Ultra-Thin Sectioning and Staining
  • 02:05Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages
  • 05:25Analyze the TEM images with ImageJ to Document Ultrastructural Features
  • 07:10Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph
  • 09:19Results: Unbiased Approach of Sampling TEM Sections
  • 10:25Conclusion

Summary

Tadução automática

脳組織の電子顕微鏡による調査のための斬新なワークフローを紹介します。メソッドでは、公平な方法で神経機能を調べることができます。元素分析は、ランダム サンプリングのワークフローのほとんどを自動化スクリプトを述べる。

Vídeos Relacionados

Read Article