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Tilapia Gölü virüs kullanarak geleneksel RT-PCR ve SYBR yeşil RT-qPCR
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
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Tilapia Gölü virüs kullanarak geleneksel RT-PCR ve SYBR yeşil RT-qPCR

26,210 Views

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10:55 min

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November 10, 2018

DOI:

10.3791/58596-v

DOI:
10.3791/58596-v

•

10:55 min
November 10, 2018

•

29 Views
, ,
1Institute of Veterinary Bacteriology Vetsuisse Faculty,University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies,Kasetsart University

Summary

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Bu iletişim kuralı Tilapia Gölü virüs (TiLV) RT-PCR yöntemlerini kullanarak tilapia dokularda tanılar. Tüm yöntemi doku diseksiyon toplam RNA emme, ardından cDNA sentezi ve TiLV geleneksel PCR veya kantitatif PCR dsDNA bir floresan bağlama boya bağlama kullanarak tarafından algılanması için tanımlanır.

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Cite this Article

Nicholson, P., Rawiwan, P., Surachetpong, W. Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR. J. Vis. Exp. (141), e58596, doi:10.3791/58596 (2018).

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