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Detectie van Tilapia Lake Virus met behulp van conventionele rechts-PCR en SYBR groene RT-qPCR
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JoVE Journal Ambiente
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
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Detectie van Tilapia Lake Virus met behulp van conventionele rechts-PCR en SYBR groene RT-qPCR

26,210 Views

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10:55 min

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November 10, 2018

DOI:

10.3791/58596-v

DOI:
10.3791/58596-v

•

10:55 min
November 10, 2018

•

29 Views
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1Institute of Veterinary Bacteriology Vetsuisse Faculty,University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies,Kasetsart University

Summary

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Dit protocol diagnose Tilapia Lake Virus (TiLV) in tilapia weefsels met behulp van de RT-PCR methodes. De hele methode is beschreven van weefsel dissectie totaal RNA extractie, gevolgd door de cDNA synthese en opsporing van TiLV door conventionele PCR of kwantitatieve PCR dsDNA bindend een bindende fluorescerende kleurstof gebruiken.

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Cite this Article

Nicholson, P., Rawiwan, P., Surachetpong, W. Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR. J. Vis. Exp. (141), e58596, doi:10.3791/58596 (2018).

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