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常规 rt-pcr 和 sybr 绿色 rt-qpcr 检测罗非鱼
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
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常规 rt-pcr 和 sybr 绿色 rt-qpcr 检测罗非鱼

26,210 Views

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10:55 min

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November 10, 2018

DOI:

10.3791/58596-v

DOI:
10.3791/58596-v

•

10:55 min
November 10, 2018

•

29 Views
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1Institute of Veterinary Bacteriology Vetsuisse Faculty,University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies,Kasetsart University

Summary

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该协议使用 rt-pcr 方法诊断罗非鱼组织中的罗非鱼病毒 (tilv)。整个方法描述了从组织解剖到总 rna 提取, 然后是 cdna 合成和检测 tilv 的常规 pcr 或定量 pcr 使用 dsdna 结合染料。

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Cite this Article

Nicholson, P., Rawiwan, P., Surachetpong, W. Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR. J. Vis. Exp. (141), e58596, doi:10.3791/58596 (2018).

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