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In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
JoVE Journal
Neurociência
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JoVE Journal Neurociência
In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

DOI:
10.3791/58570-v

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09:49 min

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November 20, 2018

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, ,
1Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Capítulos

  • 00:04Título
  • 00:45Continuous Mode ThT Aggregation Assay on a Multi-Mode Microplate Reader
  • 06:43Results: In Vitro Assay to Characterize Tau Protein Aggregation
  • 08:50Conclusion

Summary

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Tadução automática

The tau aggregation assay described in this manuscript mimics the anticipated features of in vivo tau misfolding and aggregation.

Tags

Keywords: Tau ProteinAlzheimer’s DiseaseTauopathiesAggregation AssayDrug ScreeningNucleation-dependent PolymerizationRecombinant TauFluorescenceSEC-MALSHeparinThioflavin-TMicroplate Reader

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