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Geautomatiseerde kwantificering van neutrofiele extracellulaire traps met behulp van NETQUANT
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
DOI:

07:33 min

November 27, 2019

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Capítulos

  • 00:04Título
  • 00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
  • 02:19Segmentation of Cells in the DNA Channel and the NET Channel
  • 03:41Single-cell Analysis of Identifiable Properties
  • 04:19Comparison of Cell Properties to Identify NET-forming Cells
  • 05:23Result Outputs Demeanor
  • 06:16Results: Analysis of NET Formation
  • 07:07Conclusion

Summary

Tadução automática

Hier presenteren we een protocol voor het genereren van neutrofiele extracellulaire vallen (netten) en het bedienen van NETQUANT, een volautomatische software optie voor het kwantificeren van netten in immunofluorescentie beelden.

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