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免疫荧光共聚焦显微镜对单纯疱疹病毒1蛋白核细胞质转移的时间分析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
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免疫荧光共聚焦显微镜对单纯疱疹病毒1蛋白核细胞质转移的时间分析

DOI:
10.3791/58504-v

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06:40 min

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November 04, 2018

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,
1Department of Biological Sciences,Wayne State University

Capítulos

  • 00:04Título
  • 00:31Cell Seeding, Virus Infection, and Immunofluorescent Labeling
  • 03:12Confocal Imaging and Nuclear vs. Cytoplasmic Distribution Analysis
  • 05:12Results: Representative Infected Cell Protein 0 (ICP0) Distribution Analyses
  • 05:56Conclusion

Summary

Tadução automática

Tadução automática

icp0 在1型单纯疱疹病毒感染期间经历核细胞质转移。这个事件的分子机制尚不清楚。本文介绍了共聚焦显微镜作为量化1型单纯疱疹病毒感染 icp0 运动的工具, 为定量分析 icp0 在未来的机械研究中的移位奠定了基础。

Tags

Temporal AnalysisNuclear-to-cytoplasmic TranslocationHerpes Simplex Virus 1 ProteinImmunofluorescent Confocal MicroscopyProtein TraffickingLight ImagingVirus InfectionImmunofluorescent StainingParaformaldehydePermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyDAPI

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