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Biologia
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High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
JoVE Journal
Biologia
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JoVE Journal Biologia
High-throughput Measurement of Gut Transit Time Using Larval Zebrafish

High-throughput Measurement of Gut Transit Time Using Larval Zebrafish

DOI:
10.3791/58497-v

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06:48 min

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October 23, 2018

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, ,
1Preclinical Safety,AbbVie, 2Estatística,AbbVie, 3Integrated Science and Technology,AbbVie

Capítulos

  • 00:04Título
  • 01:27Training the Larvae to Feed Using Non-dyed Food
  • 02:10Preparing Fluorescent Food, Feeding the Larvae, and Applying Treatments
  • 04:08Measuring Initial and Subsequent Fluorescence from Each Well
  • 05:04Results: High-throughput Measurement of Gut Transit Time
  • 06:22Conclusion

Summary

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The goal of this protocol is to measure the transit time of fluorescently labeled food through the gut of larval zebrafish in a high throughput fashion.

Tags

Keywords: Gut Transit TimeLarval ZebrafishHigh-throughput MeasurementFluorescent LabelFecal MatterPlate SpectrophotometerGastrointestinal BiologyGene ExpressionEnvironmental ChallengesGut FunctionTraditional MethodsFeedingRinsingTransferFluorescent FoodTest Compound

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