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电子顺磁共振在生物样品环境温度和 77 k 的应用

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

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电子顺磁共振在生物样品环境温度和 77 k 的应用

8,839 Views

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06:45 min

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January 11, 2019

DOI:

10.3791/58461-v

DOI:

10.3791/58461-v

•

06:45 min

January 11, 2019

•

3 Views

Hanan B. Elajaili¹, Laura Hernandez-Lagunas¹, Kalina Ranguelova², Sergey Dikalov³, Eva Nozik-Grayck¹

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics,University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center

Summary

电子顺磁共振 (epr) 光谱是一种明确的方法来测量自由基。使用选择性自旋探针可以检测不同细胞隔间中的自由基。我们提出了一个实用、有效的方法来收集生物样本, 方便处理、储存和转移用于 epr 测量的样品。

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