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自由运动大鼠脑电图记录过程中兴奋氨基酸的微透析

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

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自由运动大鼠脑电图记录过程中兴奋氨基酸的微透析

DOI:

10.3791/58455-v

•

08:47 min

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November 08, 2018

•

Marie Soukupová, Chiara Falcicchia, Francesca Lovisari, Selene Ingusci, Mario Barbieri, Silvia Zucchini, Michele Simonato

¹Department of Medical Sciences, Section of Pharmacology, Neuroscience Center,University of Ferrara and National Institute of Neuroscience

Capítulos

00:04Título
00:42Epileptic Behavior Monitoring
01:52Microdialysis Preparation
03:54Microdialysis: Collection of Samples
06:46Results: Extracellular Concentrations of Glutamate are Higher in Chronic Epileptic Rats
07:34Conclusion

Summary

Tadução automática

在这里, 我们描述了一种方法, 在体内微透析分析天冬氨酸和谷氨酸释放在腹侧海马的癫痫和非癫痫大鼠, 结合脑电图记录。天冬氨酸和谷氨酸的细胞外浓度可能与疾病的不同阶段有关。

Tags

Microdialysis Excitatory Amino Acids EEG Freely Moving Rats Neurotransmitter Release Neurological Diseases Faraday Cage EEG Amplifier EEG Software EEG Signal Tethered EEG Recording System Amplification Factor Ringer’s Solution FEP Tubing Guide Cannula Plexiglass Cylinder

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