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En celle-baserede analyse at undersøge ikke-muskel Myosin II kontraktilitet via foldet gastrulation signalering Pathway i Drosophila S2R + celler
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Capítulos
- 00:04Título
- 01:10Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells
- 02:25Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay
- 03:16Fixing and Staining of Constricted S2R+ Cells
- 05:18Imaging and Quantification of the Cellular Contractility Assay
- 05:41Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction
- 06:40Conclusion
Her beskriver vi en kontraktilitet analyse ved hjælp af Drosophila S2R + celler. Anvendelsen af en udefrakommende ligand, foldede gastrulation (tåge), fører til aktivering af tågen signalering pathway og cellulære kontraktilitet. Denne analyse kan bruges til at undersøge regulering af cellulære kontraktilitet proteiner i tågen signalering pathway.
