Journal
/
Biologia do Desenvolvimento
/
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

DOI:
10.3791/58325-v

•

07:15 min

•

August 19, 2018

•

, , , , , , ,
1Department of Biology,The University of North Carolina at Chapel Hill, 2Department of Biology,Reed College, 3Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

Capítulos

  • 00:04Título
  • 01:10Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells
  • 02:25Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay
  • 03:16Fixing and Staining of Constricted S2R+ Cells
  • 05:18Imaging and Quantification of the Cellular Contractility Assay
  • 05:41Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction
  • 06:40Conclusion

Summary

Tadução automática

Tadução automática

Here we describe a contractility assay using Drosophila S2R+ cells. The application of an exogenous ligand, folded gastrulation (Fog), leads to the activation of the Fog signaling pathway and cellular contractility. This assay can be used to investigate the regulation of cellular contractility proteins in the Fog signaling pathway.

Tags

Keywords: Non-muscle Myosin IIContractilityFolded-gastrulation Signaling PathwayDrosophila S2R+ CellsCell-based AssayCell DensityCell MorphologyCell Shape ChangeMorphogenesisDevelopmentGene DiscoveryRNAi Depletion

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58325?article_type=v

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
check_url/pt/58325?article_type=v

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
check_url/pt/58325?article_type=v

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
check_url/pt/58325?article_type=v

Building Finite Element Models to Investigate Zebrafish Jaw Biomechanics
check_url/pt/58325?article_type=v

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
check_url/pt/58325?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/pt/58325?article_type=v

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
check_url/pt/58325?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
check_url/pt/58325?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/pt/58325?article_type=v

HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

Read Article