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多量ホモ測定器と無料、フリー、オープン ソースの明るさ解析ソフトウェアを使用しての体外校正無料定量化
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Bioquímica
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JoVE Journal Bioquímica
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
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多量ホモ測定器と無料、フリー、オープン ソースの明るさ解析ソフトウェアを使用しての体外校正無料定量化

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08:22 min

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July 17, 2018

DOI:

10.3791/58157-v

DOI:
10.3791/58157-v

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08:22 min
July 17, 2018

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, , , , ,
1Cellular Imaging Group, Wellcome Centre Human Genetics,University of Oxford, 2Division of Structural Biology, Wellcome Centre Human Genetics,University of Oxford, 3Dynamic Structural Virology Group,Biocruces Health Research Centre, 4IKERBASQUE,Basque Foundation for Science

Tags

Protein Homo-oligomerizationIn Vitro QuantificationCalibration-freeConfocal MicroscopyBrightness AnalysisProtein-protein InteractionsDrug ScreeningCancer DrugsSmall-molecule InhibitorsFusion InhibitorsProtein DynamicsCell-cell InteractionsFKBP12His6 TagMVenus TagIMACSize-exclusion Chromatography

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Nolan, R., Alvarez, L. A., Griffiths, S. C., Elegheert, J., Siebold, C., Padilla-Parra, S. Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software. J. Vis. Exp. (137), e58157, doi:10.3791/58157 (2018).

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