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Large-scale tarımı plaka tabanlı Caenorhabditis elegans: diyabet metabolik değişiklikler çalışma için numune hazırlama
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Bioquímica
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JoVE Journal Bioquímica
Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
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Large-scale tarımı plaka tabanlı Caenorhabditis elegans: diyabet metabolik değişiklikler çalışma için numune hazırlama

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05:08 min

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August 24, 2018

DOI:

10.3791/58117-v

DOI:
10.3791/58117-v

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05:08 min
August 24, 2018

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15th Medical Department, Medical Faculty Mannheim,Heidelberg University, 2Department of Internal Medicine,Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

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Caenorhabditis ElegansPlate-based CultivationLarge-scale CultivationNematode PopulationSynchronized PopulationM9 BufferSample PreparationMetabolic AlterationsDiabetesMicroscopeDensity EvaluationPipet TipsShear StressLiquid NitrogenCentrifugationSample WashingSnap Freezing

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Kohl, K., Fleming, T., Acunman, K., Hammes, H., Morcos, M., Schlotterer, A. Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes. J. Vis. Exp. (138), e58117, doi:10.3791/58117 (2018).

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