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थाली आधारित Caenorhabditis एलिगेंसकी बड़े पैमाने पर खेती: मधुमेह में चयापचय परिवर्तन के अध्ययन के लिए नमूना तैयारी

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

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थाली आधारित Caenorhabditis एलिगेंसकी बड़े पैमाने पर खेती: मधुमेह में चयापचय परिवर्तन के अध्ययन के लिए नमूना तैयारी

6,172 Views

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05:08 min

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August 24, 2018

DOI:

10.3791/58117-v

DOI:

10.3791/58117-v

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05:08 min

August 24, 2018

•

1 Views

Katharina Kohl, Thomas Fleming³, Kübra Acunman, Hans-Peter Hammes⁴, Michael Morcos*¹, Andrea Schlotterer*¹

¹5th Medical Department, Medical Faculty Mannheim,Heidelberg University, ²Department of Internal Medicine,Heidelberg University, ³German Center for Diabetes Research (DZD), ⁴European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

Tags

Caenorhabditis Elegans Plate-based Cultivation Large-scale Cultivation Nematode Population Synchronized Population M9 Buffer Sample Preparation Metabolic Alterations Diabetes Microscope Density Evaluation Pipet Tips Shear Stress Liquid Nitrogen Centrifugation Sample Washing Snap Freezing

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