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Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
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JoVE Journal Bioquímica
Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

DOI:
10.3791/58117-v

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05:08 min

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August 24, 2018

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, , , , ,
15th Medical Department, Medical Faculty Mannheim,Heidelberg University, 2Department of Internal Medicine,Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

Capítulos

  • 00:04Título
  • 00:27Large Scale Culture on Plates
  • 01:15Transfer of Large Amounts of Caenorhabditis elegans
  • 01:52Harvest of a Large Caenorhabditis elegans Sample
  • 03:20Protein Isolation for Mass Spectrometry
  • 03:56Results: Reproducible Quantitative Protein Isolation and Further Applications of Large-scale C. elegans Culture
  • 04:47Conclusion

Summary

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This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.

Tags

Caenorhabditis ElegansPlate-based CultivationLarge-scale CultivationNematode PopulationSynchronized PopulationM9 BufferSample PreparationMetabolic AlterationsDiabetesMicroscopeDensity EvaluationPipet TipsShear StressLiquid NitrogenCentrifugationSample WashingSnap Freezing

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