Journal
/
Imunologia e Infecção
/
Isolering och identifiering av extravaskulära immunceller i hjärtat
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Isolation and Identification of Extravascular Immune Cells of the Heart
Please note that all translations are automatically generated. Click here for the English version.

Isolering och identifiering av extravaskulära immunceller i hjärtat

11,987 Views

•

08:24 min

•

August 23, 2018

DOI:

10.3791/58114-v

DOI:
10.3791/58114-v

•

08:24 min
August 23, 2018

•

21 Views
, ,
1Toronto General Hospital Research Institute,University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology,University of Toronto, 3Dept of Immunology,University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research

Summary

Automatically generated

Detta protokoll presenterar en enkel och effektiv metod för att isolera, identifiera och kvantifiera immunceller som är bosatta i myokardiet möss under steady-state eller inflammation. Protokollet kombinerar enzymatisk och mekanisk matsmältningen för generering av en enda cellsuspension som ytterligare kan analyseras med flödescytometri.

Explore More Videos

Immune CellsHeartImmunophenotypingCardiac Immune Cell PopulationsTail Vein InjectionFluorophore-conjugated Anti-CD45 AntibodyHeart PerfusionIntravascular Contaminants

Read Article

Cite this Article

Aronoff, L., Epelman, S., Clemente-Casares, X. Isolation and Identification of Extravascular Immune Cells of the Heart. J. Vis. Exp. (138), e58114, doi:10.3791/58114 (2018).

Download .ris file

Copy

Share Video

.

Copy