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Avaliação das respostas de células T Zika vírus específicos em órgãos Immunoprivileged de infectados Ifnar1- / - ratos
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1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences,China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science,Chinese Academy of Sciences, 5Laboratory Animal Center,Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences
Capítulos
- 00:04Título
- 01:15Zika Virus (ZIKV) Infection, Immunization, and Splenic Monocyte Isolation
- 03:19Brain and Testicular Monocyte Isolation
- 06:10Flow Cytometry
- 07:26Results: ZIKV Infection Characterization in Ifnar1-/- Mice
- 09:10Conclusion
Um protocolo para avaliar as respostas de células T antígeno-específicas nos órgãos immunoprivileged do Ifnar1- / - modelo murino para a infecção de vírus (ZIKV) Zika é descrito. Este método é essencial para investigar os mecanismos celulares da proteção e imunopatogênese das vacinas ZIKV e também é valioso para sua avaliação de eficácia.
Tags
Keywords:
Zika VirusT-cell ResponsesImmunoprivileged OrgansIfnar1-/- MiceTetramer StainingImmunopathogenesisGuillain-Barre SyndromeTestes InjuryInterferon Alpha/beta Receptor KnockoutBrainTestesSpleenFocus-forming UnitsRetro-orbital InoculationAbdominal SurgerySingle-cell SuspensionRed Blood Cell LysisFlow Cytometry
