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In Vitro Canine neutrofiele extracellulaire Trap vorming: Dynamische en kwantitatieve analyse door fluorescentie microscopie
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
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In Vitro Canine neutrofiele extracellulaire Trap vorming: Dynamische en kwantitatieve analyse door fluorescentie microscopie

8,523 Views

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09:45 min

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August 24, 2018

DOI:

10.3791/58083-v

DOI:
10.3791/58083-v

•

09:45 min
August 24, 2018

•

4 Views
,
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Summary

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We beschrijven de methoden om te isoleren canine neutrofielen van volbloed en visualiseren van netto vorming in levende neutrofiele granulocyten met behulp van fluorescentie microscopie. Ook beschreven zijn protocollen te kwantificeren netto vorming en citrullinated Histon H3 (citH3)-expressie met immunofluorescentie microscopie.

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Cite this Article

Li, R. H., Tablin, F. In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy. J. Vis. Exp. (138), e58083, doi:10.3791/58083 (2018).

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