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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离

8,238 Views

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07:21 min

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July 29, 2018

DOI:

10.3791/58061-v

DOI:
10.3791/58061-v

•

07:21 min
July 29, 2018

•

5 Views
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1Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Research Division of Immunology,Cedars-Sinai Medical Center

Summary

Automatically generated

我们展示了如何用磁性和荧光分选 (mac 和流动力学) 相结合的方法, 从小鼠骨髓中识别和分离出6个髓样祖细胞。本协议可用于体外培养测定 (纤维素或液体培养),体内移植实验, RNA/蛋白质分析。

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Myeloid ProgenitorsBone MarrowMACSLineage-negative CellsFACSNeutrophilsMonocytesDendritic CellsCell IsolationHematologyImmunology

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Cite this Article

Yáñez, A., Goodridge, H. S. Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow. J. Vis. Exp. (137), e58061, doi:10.3791/58061 (2018).

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