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荧光标记蛋白 ImageJ 的无偏分析在果蝇变性定量细胞生物学中的应用
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08:44 min
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August 03, 2018
DOI:
1Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University
Summary
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我们开发了一个简单且适应性强的工作流, 从荧光成像的细胞生物学研究中提取定量数据自噬性果蝇模型中中枢神经系统蛋白质聚集和通量变性.
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Quantitative Cell BiologyNeurodegenerationDrosophilaFluorescently Tagged ProteinsImageJImage Analysis WorkflowProteinaceous PunctaMembrane-bound CompartmentsNeurodegenerative DiseasesLamina DissectionRetina RemovalBrain DissectionImmunostainingAntibody IncubationMountingMicroscopyFluorescence Imaging
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Cite this Article
Brazill, J. M., Zhu, Y., Li, C., Zhai, R. G. Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ. J. Vis. Exp. (138), e58041, doi:10.3791/58041 (2018).
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