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吞噬细胞内复制脓肿分枝杆菌毒力标志物的鉴定

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

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吞噬细胞内复制脓肿分枝杆菌毒力标志物的鉴定

DOI:

10.3791/57766-v

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08:34 min

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September 27, 2018

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Violaine Dubois*¹, Laura Laencina*¹, Anouchka Bories, Vincent Le Moigne, Alexandre Pawlik, Jean-Louis Herrmann, Fabienne Girard-Misguich

¹2I, UVSQ, INSERM UMR1173,Université Paris-Saclay, ²Institut Pasteur,Unité de Pathogénomique Mycobactérienne

Capítulos

00:04Título
00:46Amoebae: M. Abscessus Tn Mutant Co-culture
03:32Intracellular Mycobacteria RNA Extraction for RNAseq Analysis
07:18Results: Mycobacterial mRNA Extraction Analysis
07:47Conclusion

Summary

Tadução automática

在这里, 我们提出两个协议来研究吞噬-分枝杆菌脓肿的相互作用: 座子突变文库的筛选和 RNA 中细菌细胞内转录的测定测序。这两种方法都提供了对基因组优势和 transcriptomic 适应增强细胞内细菌适应性的洞察力。

Tags

Mycobacterium Abscessus Virulence Markers Intracellular Replication Phagocytes Mutant Library Screening Transcriptomic Studies Acanthamoeba Castellani Amoeba Co-culture Amikacin Escherichia Coli Cell Lysis Columbia Agar Plates

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