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صبغ FM ركوب الدراجات في المشبك: مقارنة Depolarization البوتاسيوم عالية، الكهربائية وتحفيز تشانيلرهودوبسين

JoVE Journal
Neurociência

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JoVE Journal Neurociência

FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation

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صبغ FM ركوب الدراجات في المشبك: مقارنة Depolarization البوتاسيوم عالية، الكهربائية وتحفيز تشانيلرهودوبسين

8,082 Views

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08:31 min

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May 24, 2018

DOI:

10.3791/57765-v

DOI:

10.3791/57765-v

•

08:31 min

May 24, 2018

•

7 Views

Danielle L. Kopke, Kendal Broadie

¹Department of Biological Sciences,Vanderbilt University, ²Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development,Vanderbilt University and Medical Center

Tags

FM Dye Synaptic Vesicle Cycling Neuromuscular Junction Drosophila Larvae High Potassium Depolarization Electrical Stimulation Optogenetics Anti-HRP Antibody Elastomer-coated Coverslip Dissection Gluing Motor Nerve Calcium-free Saline Suction Pipette Microelectrode Puller Micro Forge Micromanipulator

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