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FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation
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Neurociência
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JoVE Journal Neurociência
FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation

FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation

8,082 Views

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08:31 min

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May 24, 2018

DOI:

10.3791/57765-v

DOI:
10.3791/57765-v

•

08:31 min
May 24, 2018

•

7 Views
,
1Department of Biological Sciences,Vanderbilt University, 2Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development,Vanderbilt University and Medical Center

Tags

FM DyeSynaptic Vesicle CyclingNeuromuscular JunctionDrosophila LarvaeHigh Potassium DepolarizationElectrical StimulationOptogeneticsAnti-HRP AntibodyElastomer-coated CoverslipDissectionGluingMotor NerveCalcium-free SalineSuction PipetteMicroelectrode PullerMicro ForgeMicromanipulator

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Kopke, D. L., Broadie, K. FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation. J. Vis. Exp. (135), e57765, doi:10.3791/57765 (2018).

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