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原位近接结扎法检测蛋白异构体的 Heterodimerization
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JoVE Journal Bioquímica
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
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原位近接结扎法检测蛋白异构体的 Heterodimerization

7,309 Views

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09:18 min

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October 20, 2018

DOI:

10.3791/57755-v

DOI:
10.3791/57755-v

•

09:18 min
October 20, 2018

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2 Views
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1Fox Chase Cancer Center

Tags

Protein IsoformsIn Situ Proximity Ligation AssayPLAProtein InteractionsHeterodimersMST1MST2Fluorescence SignalMicroscope ImagingCell CultureCell FixationCell Permeabilization

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Cite this Article

Karchugina, S., Chernoff, J. Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay. J. Vis. Exp. (140), e57755, doi:10.3791/57755 (2018).

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