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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
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JoVE Journal Bioquímica
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

DOI:
10.3791/57755-v

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09:18 min

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October 20, 2018

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,
1Fox Chase Cancer Center

Capítulos

  • 00:02Título
  • 02:19Fixation, Permeabilization and Blocking of Cells
  • 03:29Primary Antibodies
  • 04:15PLA Probes
  • 04:34Ligation
  • 05:27Amplification
  • 06:19Preparation for Imaging
  • 07:31Resultados
  • 08:40Conclusion

Summary

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Here, we show how to use a Proximity Ligation Assay (PLA) to visualize MST1/MST2 heterodimerization in fixed cells with high sensitivity.

Tags

Protein IsoformsIn Situ Proximity Ligation AssayPLAProtein InteractionsHeterodimersMST1MST2Fluorescence SignalMicroscope ImagingCell CultureCell FixationCell Permeabilization

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