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毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

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毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析

DOI:

10.3791/57749-v

•

07:54 min

•

August 22, 2018

•

Markus Nachbar, Jorrit Maul, Matthias Stein, Hermann Wätzig

¹Institute of Medicinal and Pharmaceutical Chemistry,TU Braunschweig

Capítulos

00:04Título
00:34Preparation of Capillaries
01:45Vial Preparation and Affinity Capillary Electrophoretic Separation
03:34Affinity Capillary Electrophoretic Analysis – Preparation for ACE Methods
05:20Results: Characterization of AtHIRD11 by Affinity Capillary Electrophoresis and Capillary Gel Electrophoresis
06:59Conclusion

Summary

Tadução automática

该协议结合毛细管电泳对蛋白质样品的特性进行了表征, 并对带电配体进行了快速结合筛选。推荐用于具有弹性结构的蛋白质, 如内在紊乱的蛋白质, 以确定不同构象的结合的差异。

Tags

Keywords: Intrinsic Disorder Metal Ion Binding Capillary Gel Electrophoresis Affinity Capillary Electrophoresis Conformational Changes Glass Capillary Polyamide Coating Detection Window Calcium Chloride Metal Salt Solutions Tris Buffer Acetanilide EOF Marker Sample Solution Hydrodynamic Injection Electrophoretic Separation

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