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微操作系统技术允许分析骨架调节器的形态发生动力学和营业额

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

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微操作系统技术允许分析骨架调节器的形态发生动力学和营业额

DOI:

10.3791/57643-v

•

12:52 min

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May 12, 2018

•

Georgi Dimchev², Klemens Rottner²

¹Division of Molecular Cell Biology, Zoological Institute,Technische Universität Braunschweig, ²Department of Cell Biology,Helmholtz Centre for Infection Research

Capítulos

00:05Título
00:50Treatment of Cells, Transfection, and Seeding onto Coverslips
02:54Assembly of Microscopy Imaging Chamber
03:43Microinjection Procedure
05:02FRAP Procedure
05:32Photoactivation Procedure
06:26Data Analysis
09:42Results: Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
11:27Conclusion

Summary

Tadução automática

我们描述了微和 photomanipulation 技术, 如酶和 photoactivation 如何能够确定运动参数和蛋白质在迁移细胞内的时空动力学。实验读数包括亚细胞动力学和运动调节器的更替或底层肌动蛋白骨架。

Tags

Keywords: Micromanipulation Cytoskeletal Regulators FRAP Photoactivation B16-F1 Cells NIH 3T3 Fibroblasts Laminin Fibronectin Transfection Microscopy

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