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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
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Medicina
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JoVE Journal Medicina
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

DOI:
10.3791/57637-v

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09:51 min

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May 18, 2018

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, ,
1Department of Biochemistry, School of Medicine,Case Western Reserve University, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology,Case Western Reserve University, 3Angie Fowler Cancer Institute, Rainbow Babies & Children’s Hospital,University Hospitals, Cleveland

Capítulos

  • 00:04Título
  • 01:05Bone Marrow-derived Dendritic Cell (BMDC) Preparation
  • 03:25T Cell Syngeneic Proliferation Assay
  • 07:26Results: Representative Tolerogenic DC (TolDC) Analyses
  • 08:53Conclusion

Summary

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Tadução automática

Here, we present a protocol to develop and characterize tolerogenic dendritic cells (TolDCs) and evaluate their immunotherapeutic utility.

Tags

Keywords: MurineTolerogenicDendritic CellsAutoimmune DisordersMultiple SclerosisImmunotherapyCell DevelopmentFunctional CharacterizationC57BL/6 MiceBone MarrowGM-CSFIL-4Cell CultureCell HarvestingCell LysisCell FilteringCell PlatingCell FeedingCell Incubation

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