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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites
 

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Article DOI: 10.3791/57486-v • 10:01 min • March 16th, 2018
March 16th, 2018 •
1, 1, 1, 1, 1
1Department of Cell Biology and Molecular Genetics, University of Maryland

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All pathogenic Leishmania species reside and replicate inside macrophages of their vertebrate hosts. Here, we present a protocol to infect murine bone marrow-derived macrophages in culture with Leishmania, followed by precise quantification of intracellular growth kinetics. This method is useful for studying individual factors influencing host-pathogen interaction and Leishmania virulence.

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Keywords: Macrophage Leishmania Virulence Intracellular Growth Bone Marrow-derived In Vitro Murine Parasite Quantification Therapy Leishmaniasis Mutated Strains Therapeutic Compounds Isolation Dissection
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