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Biologia do Desenvolvimento
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Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

DOI:
10.3791/57330-v

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08:55 min

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April 25, 2018

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1Department of Comparative Pathobiology,Purdue University, 2Purdue University Center for Cancer Research, Purdue Institute for Inflammation, Immunology and Infectious Diseases (PI4D), Purdue Institute for Integrative Neuroscience (PIIN),Purdue University

Capítulos

  • 00:05Título
  • 00:46Microinjection
  • 05:24Imaging
  • 06:52Results: Visualization of Embryonic Development, Movement, and Tumor Cells In Vivo
  • 08:11Conclusion

Summary

Tadução automática

Tadução automática

Here, we show the process of creating a cellular electric voltage reporter zebrafish line to visualize embryonic development, movement, and fish tumor cells in vivo.

Tags

ZebrafishEmbryogenesisCellular Electrical ActivityTumorIn VivoReal-time ImagingTol2 TransposaseMRNA InjectionEmbryo CollectionAgarose Injection MoldMicroinjection

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