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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolating Lymphocytes from the Mouse Small Intestinal Immune System
 

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Article DOI: 10.3791/57281-v • 11:28 min • February 28th, 2018
February 28th, 2018 •
1, 1
1Center for Infectious Diseases, Department of Molecular Genetics and Microbiology, Stony Brook University

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Resumo
Automatic Translation

Here we describe a detailed protocol for the isolation of lymphocytes from the inductive sites including the gut-associated lymphoid tissue Peyer's patches and the draining mesenteric lymph nodes, and the effector sites including the lamina propria and the intestinal epithelium of the small intestinal immune system.

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Isolating Lymphocytes Mouse Small Intestinal Immune System Peyer's Patches Mesenteric Lymph Nodes Lamina Propria Epithelium Mucosal Immunology Gastrointestinal Infections Cancers Inflammatory Diseases Highly Purified Lymphocytes Minimal Cross-compartmental Contamination Mesenteric Lymph Nodes Identification And Isolation Peyer's Patches Identification And Isolation Optimal Yields Midline Incision Peritoneal Cavity Exposure Cecum Location And Manipulation Mesenteric Lymph Nodes (MLN) Chain Identification And Movement
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