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Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
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Neurociência
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JoVE Journal Neurociência
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

14,285 Views

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09:44 min

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July 10, 2018

DOI:

10.3791/57270-v

DOI:
10.3791/57270-v

•

09:44 min
July 10, 2018

•

3 Views
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1Institute of Neuroscience,National Yang-Ming University, 2Brain Research Center,National Yang-Ming University

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Stereotaxic SurgeryGenetic ManipulationStriatal CellsNeonatal Mouse BrainsPostnatal DevelopmentVisual DemonstrationHome-made DeviceTargeted InjectionsHead TrayStainless Steel NeedlesChloroformEthanolMicroinjection AssemblyPE20 Polyethylene TubingPE10 Tubing26 Gauge Syringe30 Gauge Injection Needle10 Microliter Microinjection Syringe25 Gauge Syringe0.1% Fast Green DyeFiltered Solution

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Chen, S., Kuo, H., Liu, F. Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains. J. Vis. Exp. (137), e57270, doi:10.3791/57270 (2018).

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