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用高频超声 (30/45 mhz) 系统对胎儿小鼠进行心血管成像
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System
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用高频超声 (30/45 mhz) 系统对胎儿小鼠进行心血管成像

DOI:
10.3791/57210-v

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07:34 min

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May 05, 2018

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1Neonatal/Congenital Heart Laboratory, Cardiovascular Research Laboratory, David Geffen School of Medicine,University of California, Los Angeles, 2Children’s Discovery and Innovation Institute, Department of Pediatrics, David Geffen School of Medicine,University of California, Los Angeles

Capítulos

  • 00:05Título
  • 00:45Preparing the High Frequency Ultrasound Imaging System
  • 01:36Preparing the Pregnant Mouse
  • 02:35Embryo Identification
  • 03:09Fetal Heart Visualization and Annotation
  • 05:05Evaluating Cardiopulmonary Flow Parameters
  • 05:44Evaluating the Feto-placental Axis
  • 06:30Results: Hemodynamic Parameters of Normoxic and Hypoxic Fetal Mice at Gestational Day 18.5
  • 06:56Conclusion

Summary

Tadução automática

Tadução automática

高频超声成像的胎儿小鼠有改进的影像分辨率, 可以提供精确的非侵入性的心脏发育和结构缺陷的特点。此处概述的协议旨在执行实时的胎儿小鼠超声心动图在体内。

Tags

Fetal MouseCardiovascular ImagingHigh-frequency UltrasoundCardiac MorphologyFlow IndicesCongenital Heart Defects30/45MHz2D Dynamic ImagingAnesthesiaElectrocardiographyTemperature MonitoringFur RemovalFetal Orientation

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