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解離性個体の親和性の浄化 (BiCAP) によって多蛋白質シグナリング複合体
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06:45 min
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June 15, 2018
DOI:
1The Kinghorn Cancer Centre,Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences,University of Copenhagen, 3RCSI Molecular Medicine,Royal College of Surgeons in Ireland, 4Department of Epigenetics,Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences,University of New South Wales, 6St Vincent’s Hospital Clinical School,University of New South Wales, 7School of Medicine and Medical Science,University College Dublin
Summary
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本稿では、二分子の相補性の親和性の浄化 (BiCAP) のプロトコルについて説明します。この手法は競合結合パートナーで形成された複合体と同様、国連複合体の個々 のタンパク質を除く、特定の分離と任意の 2 つの相互作用の蛋白質の下流のプロテオーム解析を促進します。
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Hastings, J. F., Han, J. Z., Shearer, R. F., Kennedy, S. P., Iconomou, M., Saunders, D. N., Croucher, D. R. Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP). J. Vis. Exp. (136), e57109, doi:10.3791/57109 (2018).
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