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Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
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Bioquímica
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JoVE Journal Bioquímica
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

DOI:
10.3791/57070-v

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09:15 min

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August 14, 2018

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, , , , ,
1Xtal Concepts GmbH, 2Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY,University of Hamburg, 3The Hamburg Center for Ultrafast Imaging,University of Hamburg

Capítulos

  • 00:04Título
  • 00:56Parameters, Solution Setup, and Micro-dosage Systems Adjustment
  • 02:42Seeing a Thaumatin Drop in the Experimental Chamber and in Situ Measurements
  • 04:48Sample Evaporation, Precipitant Addition, and Tracking the Evolution of the Crystallization Droplet Over Time
  • 06:12Recovery of Crystallization Droplet
  • 07:09Results: Formation of Protein Microcrystals from T. daniellii Thaumatin
  • 07:57Conclusion

Summary

Tadução automática

Tadução automática

Here we present a protocol for controlled production of protein microcrystals. The process uses an automated device allowing controlled manipulation of several crystallization parameters. The protein crystallization is carried-out by controlled and automated addition of crystallization solutions while monitoring and investigating the radius distribution of particles in the crystallization droplet.

Tags

Protein CrystallizationDynamic Light ScatteringIn Situ MonitoringAutomated CrystallizationCrystal GrowthNano/micro-crystalsThaumatinSodium Tartrate

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