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Immunology and Infection
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JoVE Journal Immunology and Infection
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
 

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Article DOI: 10.3791/57061-v • 11:09 min • December 9th, 2017
December 9th, 2017 •
1,2, 3, 4, 1, 1,2
1Department of Chemistry and Biochemistry, University of Arkansas, 2Cell and Molecular Biology Program, University of Arkansas, 3Department of Biological Sciences, University of Arkansas, 4Fayetteville High School

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Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.

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Facile Protocol Site-specific Acetylation Proteins Escherichia Coli Expression Host Lysine Acetylation Post-translational Modifications Biological Processes Gene Transcription Stress Response Cellular Differentiation Metabolism Genetic Code Expansion Technique Pyrrolysyl-tRNA Synthetase Variant Acetyllysine UAG Stop Codon Suppression Target Protein Amber Stop Codon Site-directed Mutagenesis Template Plasmid PCR Reaction
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