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Imunologia e Infecção
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Evaluación de la citotóxica y efectos inmunomoduladores de sustancias en lonchas de pulmón humano de precisión de corte
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
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Evaluación de la citotóxica y efectos inmunomoduladores de sustancias en lonchas de pulmón humano de precisión de corte

13,195 Views

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12:04 min

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May 09, 2018

DOI:

10.3791/57042-v

DOI:
10.3791/57042-v

•

12:04 min
May 09, 2018

•

16 Views
, , , , , , , , , , , ,
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery,Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology,RWTH Aachen University, 6Institute for Immunology,Hannover Medical School

Summary

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Teniendo en cuenta el principio 3R, están evolucionando los modelos respiratorios como alternativas a los estudios en animales. Especialmente para la evaluación del riesgo de las sustancias respiratorias, existe una falta de ensayos adecuados. Aquí, describimos el uso de cortes de pulmón humano de precisión de corte para la evaluación de sustancias aerotransportadas.

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Neuhaus, V., Danov, O., Konzok, S., Obernolte, H., Dehmel, S., Braubach, P., Jonigk, D., Fieguth, H., Zardo, P., Warnecke, G., Martin, C., Braun, A., Sewald, K. Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices. J. Vis. Exp. (135), e57042, doi:10.3791/57042 (2018).

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