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1Department of Health Sciences,Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies,National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies,National Research Council, Bari, Italy, 4Department of Life Sciences,University of Trieste, Italy, 5Institute of Genetic and Biomedical Research,National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy
Capítulos
- 00:04Título
- 01:19Construction of the ORF Library
- 02:38Preparation of the Filtering Vector
- 03:40Ligation and Transformation
- 06:47pFILTER-ORF Library Validation and Collection
- 09:10Phage Library Deep Sequencing Platform, Index PCR Followed by Qualitative and Quantitative Evaluation of the Sequencing Library
- 10:07Results: Use of a Phagemid Vector to Construct, Characterize and Select a Domainome Library
- 11:23Conclusion
प्रोटोकॉल का वर्णन निर्माण, लक्षण वर्णन और चयन की अनुमति (पसंद के लक्ष्य के खिलाफ) एक "domainome" किसी भी डीएनए स्रोत से बना पुस्तकालय । फेज प्रदर्शन, एक तह रिपोर्टर और डेटा विश्लेषण के लिए एक वेब उपकरण के साथ अगली पीढ़ी sequencing: यह एक अनुसंधान पाइपलाइन है कि विभिन्न प्रौद्योगिकियों को जोड़ती द्वारा हासिल की है.
