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Efficiënte productie en identificatie van CRISPR/Cas9-gegenereerd Gene uitsparingen in de modelsysteem Danio rerio
JoVE Journal
Genética
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JoVE Journal Genética
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
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Efficiënte productie en identificatie van CRISPR/Cas9-gegenereerd Gene uitsparingen in de modelsysteem Danio rerio

20,970 Views

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11:27 min

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August 28, 2018

DOI:

10.3791/56969-v

DOI:
10.3791/56969-v

•

11:27 min
August 28, 2018

•

10 Views
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1Department of Biochemistry,Purdue University

Tags

CRISPR/Cas9Gene KnockoutDanio RerioZebrafishTargeted Genome EditingIn Vitro TranscriptionSgRNAAmmonium Acetate PrecipitationEthanol PrecipitationRNA Purification

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Sorlien, E. L., Witucki, M. A., Ogas, J. Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio. J. Vis. Exp. (138), e56969, doi:10.3791/56969 (2018).

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