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斑马斑马模型系统中 CRISPR/Cas9-generated 基因挖空的高效生产与鉴定
JoVE Journal
Genética
This content is Free Access.
JoVE Journal Genética
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
DOI:

11:27 min

August 28, 2018

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Capítulos

  • 00:05Título
  • 01:09Preparation of CRISPR-reagents for Embryo Microinjection
  • 04:03Microinjection of CRISPR-components into Zebrafish Embryos
  • 06:12Analysis of Efficiency of Indel Formation Using an HMA
  • 08:45Identification and Propagation of Knock-out Lines
  • 09:45Results: Zebrafish Embryos Exhibit a Pigment Defect when Injected with an sgRNA Targeting Tyrosinase at the One Cell Stage
  • 10:32Conclusion

Summary

Tadução automática

斑马斑马(斑马鱼) 在模型系统中的靶向基因组编辑由于 CRISPR 方法的出现而大大促进。在这里, 我们描述了一个简化的, 健壮的协议, 以生成和鉴定 CRISPR 衍生的无意义的等位基因, 包括 heteroduplex 移动性检测和识别突变使用下一代测序。

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