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用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学
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10:41 min
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March 07, 2018
DOI:
1Department of General Surgery, Tongren Hospital,Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine,Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital,Shanghai Jiao Tong University School of Medicine
Summary
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该协议描述了纯化 hEAG1 离子通道蛋白与小分子脂配体磷酸肌醇4、5-bisphosphate (PIP2) 的相互作用。实验表明, BLI 可能是一种新的小分子离子通道配体筛选的潜在方法。
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Cite this Article
Han, B., Zhang, M., Sun, P., Hou, S. Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay. J. Vis. Exp. (133), e56846, doi:10.3791/56846 (2018).
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