Journal
/
Genética
/
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

DOI:
10.3791/56736-v

•

10:39 min

•

February 26, 2018

•

, ,
1Department of Biochemistry and Molecular Biology,Johns Hopkins University Bloomberg School of Public Health

Capítulos

  • 00:04Título
  • 00:59Harvesting Fetal or Neonatal Ovaries
  • 03:55Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
  • 05:17Oocyte Denuding and Zona Pellucida Removal
  • 07:05MI and MII Oocyte Chromatin Spreads
  • 08:08Results: Crossover Formation in C57B/6J Oocytes
  • 09:52Conclusion

Summary

Tadução automática

Tadução automática

Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.

Tags

Keywords: Chromatin SpreadMouse OocyteProphaseMetaphase IIOogenesisChromosomeImmunolabelingPloidyAneuploidyInfertilityOvaryHypo-extraction BufferSucrose

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/56736?article_type=v

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
check_url/pt/56736?article_type=v

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
check_url/pt/56736?article_type=v

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
check_url/pt/56736?article_type=v

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
check_url/pt/56736?article_type=v

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
check_url/pt/56736?article_type=v

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
check_url/pt/56736?article_type=v

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
check_url/pt/56736?article_type=v

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
check_url/pt/56736?article_type=v

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
check_url/pt/56736?article_type=v

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Read Article